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scrambled sirna nontarget control  (Azenta)

 
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    Structured Review

    Azenta scrambled sirna nontarget control
    The role <t>of</t> <t>SMS</t> in HNSCC. (A) SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with <t>siRNA</t> sequences (Si-1 and Si-2) (B) The expression of SMS in HNSCC tissue and normal tissue of patients. t-test was used to compare the expression of genes between normal and tumor. (C, D) The results of the plate cloning assay of SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (E, F) Scratch-wound healing assay depicted that a significantly slower wound healing rate was observed in cells with a decreased expression of SMS. (G, H) Transwell assay showed that downregulation of SMS expression inhibited the migration and invasion capacity of HNSCC cells. To demonstrate the accuracy and reproducibility of the results, all experiments were repeated in two HNSCC (HN-5, UMSCC-47) cell lines and all data were presented as the means ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.
    Scrambled Sirna Nontarget Control, supplied by Azenta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scrambled+sirna+nontarget+control/scrambled+sirna+nontarget+control/pmc10982401-103-8-14
    Average 90 stars, based on 1 article reviews
    scrambled sirna nontarget control - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Comprehensive investigation into the influence of glycosylation on head and neck squamous cell carcinoma and development of a prognostic model for risk assessment and anticipating immunotherapy"

    Article Title: Comprehensive investigation into the influence of glycosylation on head and neck squamous cell carcinoma and development of a prognostic model for risk assessment and anticipating immunotherapy

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2024.1364082

    The role of SMS in HNSCC. (A) SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (Si-1 and Si-2) (B) The expression of SMS in HNSCC tissue and normal tissue of patients. t-test was used to compare the expression of genes between normal and tumor. (C, D) The results of the plate cloning assay of SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (E, F) Scratch-wound healing assay depicted that a significantly slower wound healing rate was observed in cells with a decreased expression of SMS. (G, H) Transwell assay showed that downregulation of SMS expression inhibited the migration and invasion capacity of HNSCC cells. To demonstrate the accuracy and reproducibility of the results, all experiments were repeated in two HNSCC (HN-5, UMSCC-47) cell lines and all data were presented as the means ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: The role of SMS in HNSCC. (A) SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (Si-1 and Si-2) (B) The expression of SMS in HNSCC tissue and normal tissue of patients. t-test was used to compare the expression of genes between normal and tumor. (C, D) The results of the plate cloning assay of SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (E, F) Scratch-wound healing assay depicted that a significantly slower wound healing rate was observed in cells with a decreased expression of SMS. (G, H) Transwell assay showed that downregulation of SMS expression inhibited the migration and invasion capacity of HNSCC cells. To demonstrate the accuracy and reproducibility of the results, all experiments were repeated in two HNSCC (HN-5, UMSCC-47) cell lines and all data were presented as the means ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Expressing, Clone Assay, Wound Healing Assay, Transwell Assay, Migration

    Related Articles

    Expressing:

    Article Title: Comprehensive investigation into the influence of glycosylation on head and neck squamous cell carcinoma and development of a prognostic model for risk assessment and anticipating immunotherapy
    Article Snippet: .. The SMS siRNA expression vector and the scrambled siRNA nontarget control were acquired from Genewiz (China). .. Plasmids underwent transfection utilizing Lipofectamine 3000 (Thermo Scientific, USA), following the manufacturer’s prescribed protocols.

    Control:

    Article Title: Comprehensive investigation into the influence of glycosylation on head and neck squamous cell carcinoma and development of a prognostic model for risk assessment and anticipating immunotherapy
    Article Snippet: .. The SMS siRNA expression vector and the scrambled siRNA nontarget control were acquired from Genewiz (China). .. Plasmids underwent transfection utilizing Lipofectamine 3000 (Thermo Scientific, USA), following the manufacturer’s prescribed protocols.



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    The role <t>of</t> <t>SMS</t> in HNSCC. (A) SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with <t>siRNA</t> sequences (Si-1 and Si-2) (B) The expression of SMS in HNSCC tissue and normal tissue of patients. t-test was used to compare the expression of genes between normal and tumor. (C, D) The results of the plate cloning assay of SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (E, F) Scratch-wound healing assay depicted that a significantly slower wound healing rate was observed in cells with a decreased expression of SMS. (G, H) Transwell assay showed that downregulation of SMS expression inhibited the migration and invasion capacity of HNSCC cells. To demonstrate the accuracy and reproducibility of the results, all experiments were repeated in two HNSCC (HN-5, UMSCC-47) cell lines and all data were presented as the means ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.
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    The role <t>of</t> <t>SMS</t> in HNSCC. (A) SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with <t>siRNA</t> sequences (Si-1 and Si-2) (B) The expression of SMS in HNSCC tissue and normal tissue of patients. t-test was used to compare the expression of genes between normal and tumor. (C, D) The results of the plate cloning assay of SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (E, F) Scratch-wound healing assay depicted that a significantly slower wound healing rate was observed in cells with a decreased expression of SMS. (G, H) Transwell assay showed that downregulation of SMS expression inhibited the migration and invasion capacity of HNSCC cells. To demonstrate the accuracy and reproducibility of the results, all experiments were repeated in two HNSCC (HN-5, UMSCC-47) cell lines and all data were presented as the means ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.
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    Image Search Results


    The role of SMS in HNSCC. (A) SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (Si-1 and Si-2) (B) The expression of SMS in HNSCC tissue and normal tissue of patients. t-test was used to compare the expression of genes between normal and tumor. (C, D) The results of the plate cloning assay of SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (E, F) Scratch-wound healing assay depicted that a significantly slower wound healing rate was observed in cells with a decreased expression of SMS. (G, H) Transwell assay showed that downregulation of SMS expression inhibited the migration and invasion capacity of HNSCC cells. To demonstrate the accuracy and reproducibility of the results, all experiments were repeated in two HNSCC (HN-5, UMSCC-47) cell lines and all data were presented as the means ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Comprehensive investigation into the influence of glycosylation on head and neck squamous cell carcinoma and development of a prognostic model for risk assessment and anticipating immunotherapy

    doi: 10.3389/fimmu.2024.1364082

    Figure Lengend Snippet: The role of SMS in HNSCC. (A) SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (Si-1 and Si-2) (B) The expression of SMS in HNSCC tissue and normal tissue of patients. t-test was used to compare the expression of genes between normal and tumor. (C, D) The results of the plate cloning assay of SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (E, F) Scratch-wound healing assay depicted that a significantly slower wound healing rate was observed in cells with a decreased expression of SMS. (G, H) Transwell assay showed that downregulation of SMS expression inhibited the migration and invasion capacity of HNSCC cells. To demonstrate the accuracy and reproducibility of the results, all experiments were repeated in two HNSCC (HN-5, UMSCC-47) cell lines and all data were presented as the means ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: The SMS siRNA expression vector and the scrambled siRNA nontarget control were acquired from Genewiz (China).

    Techniques: Expressing, Clone Assay, Wound Healing Assay, Transwell Assay, Migration